Molecular evaluation of selected mango genotypes grown in Egypt using SCoT and ISSR markers

https://doi.org/10.17221/112/2021-HORTSCICitation:

Ghounim I.E., Mustafa M.R., Hmmam I. (2022): Molecular evaluation of some mango genotypes using SCoT and ISSR markers. Hort. Sci. (Prague), 49: 213–224.

download PDF

The mango (Mangifera indica L.) is a common tropical and subtropical fruit and the second main fruit crop in Egypt. In this work, start codon targeted (SCoT) and inter simple sequence repeat (ISSR) markers were employed to study the genetic relationship among twenty-three mango genotypes grown under the El-Giza district conditions. These genotypes consist of four newly introduced cultivars, six new progenies and thirteen closely related mango cultivars which were included as cultivar references for the best definition. Based on the overall SCoT and ISSR data, a total of 52 bands were generated (18 for SCoT and 34 for ISSR) with a total polymorphic percentage equal to 53.84% (44.44% for SCoT and 58.82% for ISSR). Based on the whole set of SCoT and ISSR marker data, the genetic similarity coefficients ranged from 80 to 97% with a mean equal to 88.5% and the first two principal axes explained about 31% of the total genetic variation among the mango accessions. The SCoT and ISSR data revealed that the four newly introduced mango genotypes, namely ‘Aya’, ‘Kasturi’, ‘Maya’ and ‘Omer’, are novel genetic resources based on their genetic profiles, which can be used for future breeding programmes in Egypt.

References:
Abubakar B.Y., Wusirika R., MuA‘zu S., Khan A.U., Adamu A.K. (2011): Detection of genetic variability using random amplified polymorphic DNA markers in some accessions of Moringa oleifera Lam. from northern Nigeria. International Journal of Botany, 7: 237–242. https://doi.org/10.3923/ijb.2011.237.242
 
Ahmedand T.H.M., Mohamed Z.M.A. (2015): Genetic diversity of mango (Mangifera indica L.) cultivars in Shendi Area. Extensive Journal of Applied Sciences, 3: 219–224.
 
Ajayi I.I., Olawuyi O.J., Ayodele A.E., Faneye A.O. (2019): Molecular relationship among Mangifera indica L. (Mango) varieties using simple sequence repeat (SSR) marker. Journal of Advances in Biology & Biotechnology, 22: 1–16.
 
Alansi S., Tarroum M., Al-Qurainy F., Khan S., Nadeem M. (2016): Use of ISSR markers to assess the genetic diversity in wild medicinal Ziziphus spina-christi (L.) Willd. collected from different regions of Saudi Arabia. Biotechnology & Biotechnological Equipment, 30: 942–947.
 
Amirmoradi B., Talebi R., Karami E. (2012): Comparison of genetic variation and differentiation among annual Cicer species using start codon targeted (SCoT) polymorphism, DAMD-PCR, and ISSR markers. Plant Systematics and Evolution, 298: 1679–1688. https://doi.org/10.1007/s00606-012-0669-6
 
Anggraheni Y.G.D., Mulyaningsih E.S. (2021): Diversity assessment of mango (Mangifera spp) plant collection of cibinong germplasm garden based on leaves morphology and RAPD markers. In IOP Conference Series. Earth and Environmental Science, 715: 1–12.  https://doi.org/10.1088/1755-1315/715/1/012045
 
Ariffin Z., Sah M.S.M., Idris S., Hashim N. (2015): Genetic diversity of selected Mangifera species revealed by inter simple sequence repeats markers. International Journal of Biodiversity: 1–8. https://doi.org/10.1155/2015/458237
 
Azmat M.A., Khan A.A., Khan I.A., Rajwana I.A., Cheema H.M.N., Khan A.S. (2016): Morphological characterization and SSR based DNA fingerprinting of elite commercial mango cultivars. Pakistan Journal of Agricultural Sciences, 53: 321–330. https://doi.org/10.21162/PAKJAS/16.2988
 
Bhattacharyya P., Kumaria S., Kumar S., Tandon P. (2013): Start Codon Targeted (SCoT) marker reveals genetic diversity of Dendrobium nobile Lindl., an endangered medicinal orchid species. Gene, 529: 21–26. https://doi.org/10.1016/j.gene.2013.07.096
 
Collard B.C., Mackill D.J. (2009): Start codon targeted (SCoT) polymorphism: a simple, novel DNA marker technique for generating gene-targeted markers in plants. Plant Molecular Biology Reporter, 27: 86. https://doi.org/10.1007/s11105-008-0060-5
 
Crespel L., Pernet A., Le Bris M., Gudin S., Oyant L.H.S. (2009): Application of ISSRs for cultivar identification and assessment of genetic relationships in rose. Plant breeding, 128: 501–506. https://doi.org/10.1111/j.1439-0523.2008.01600.x
 
Damodaran T., Kannan R., Ahmed I., Srivastava R.C., Rai R.B., Umamaheshwari S. (2012): Assessing genetic relationships among mango (Mangifera indica L.) accessions of Andaman Islands using inter simple sequence epeat markers. New Zealand Journal of Crop and Horticultural Science, 40: 229–240. https://doi.org/10.1080/01140671.2012.662158
 
Dang, Z., Chen Y. (2017): Construction of a genetic linkage map of mango based on SRAP, AFLP and ISSR markers. Agricultural Biotechnology, 6: 9–16.
 
Díaz-Matallana M., Schuler-García I., Ruiz-García M., Hodson de Jaramillo E. (2009): Analysis of diversity among six populations of Colombian mango (Mangifera indica L. cvar. Hilacha) using RAPDs markers. Electronic Journal of Biotechnology, 12: 1–8.
 
Doyle J.J., Doyle J.L. (1987): A rapid DNA isolation procedure for small quantities of fresh leaf tissue. Phytochemical Bulletin, 19: 11–15.
 
Duval M.F., Bunel J., Sitbon C., Risterucci A.M. (2005): Development of microsatellite markers for mango (Mangifera indica). Molecular Ecology Notes, 5: 824–826. https://doi.org/10.1111/j.1471-8286.2005.01076.x
 
Eiadthong W., Yonemori K., Kanzaki S., Sugiura A., Utsunomiya N., Subhadrabandhu S. (2000): Amplified fragment length polymorphism analysis for studying genetic relationships among Mangifera species in Thailand. Journal of the American Society for Horticultural Science, 125: 160–164. https://doi.org/10.21273/JASHS.125.2.160
 
El-Sayed M.E.A., El-nawam S.M.A., Mostafa G. (2018): Evaluation of Local Mango Cultivars Grown under Different Locations in Egypt using Fruit Quality and RAPD analysis. Middle East Journal of Agriculture Research, 7: 473–480.
 
Fatimah F., Husni A., Kosmiatin M., Karsinah K., Baroya M. (2016): Characterization of zygotic and nucellar embryo of six indonesian mango cultivars using molecular markers. Annales Bogorienses, 20: 69–75.
 
Galal O., Galal H., Aboulila A. (2017): Genetic variability and molecular characterization of some local and imported mango cultivars in Egypt. Egyptian Journal of Genetics and Cytology, 46: 121–138. https://doi.org/10.21608/ejgc.2018.9509
 
Gajera H.P., Bambharolia R.P., Domadiya R.K., Patel S.V., Golakiya B.A. (2014): Molecular characterization and genetic variability studies associated with fruit quality of indigenous mango (Mangifera indica L.) cultivars. Plant Systematics and Evolution, 300: 1011–1020. https://doi.org/10.1007/s00606-013-0939-y
 
Gajera H.P., Tomar R.S., Patel S.V., Viradia R.R., Golakiya B.A. (2011): Comparison of RAPD and ISSR markers for genetic diversity analysis among different endangered Mangifera indica genotypes of Indian Gir forest region. Journal of Plant Biochemistry and Biotechnology, 20: 217–223. https://doi.org/10.1007/s13562-011-0049-2
 
Ganesan K.S., Singh R., Choudhury D.R., Bharadwaj J., Gupta V., Singode A. (2014): Genetic diversity and population structure study of drumstick (Moringa oleifera Lam.) using morphological and SSR markers. Industrial Crops and Products, 60: 316–325. https://doi.org/10.1016/j.indcrop.2014.06.033
 
Gitahi R., Kasili R., Kyallo M., Kehlenbeck K. (2016): Diversity of threatened local mango landraces on smallholder farms in Eastern Kenya. Forests, Trees and Livelihoods, 25: 239–254. https://doi.org/10.1080/14728028.2016.1201436
 
González A., Coulson M., Brettell R. (2002): Development of DNA markers (ISSRs) in mango. Acta Horticulturae (ISHS), 575: 139–143. https://doi.org/10.17660/ActaHortic.2002.575.13
 
Gorji A.M., Poczai P., Polgar Z., Taller J. (2011): Efficiency of arbitrarily amplified dominant markers (SCoT, ISSR and RAPD) for diagnostic fingerprinting in tetraploid potato. American Journal of Potato Research, 88: 226–237. https://doi.org/10.1007/s12230-011-9187-2
 
Haseeb G.M., Ghounim I.E.S., Hmmam I., Mustafa M.R. (2020): Evaluation of four newly introduced mango (Mangifera indica L.) cultivars grown under El-Giza conditions. Plant Archives, 20: 9405–9410.
 
He X.H., Li Y.R., Guo Y.Z., Ou S.J., Li R.B. (2007): Identification of closely related mango cultivars by ISSR. Guihaia, 27: 44–47.
 
He X.H., Li Y.R., Guo Y.Z., Tang Z.P., Li R.B. (2005): Genetic analysis of 23 mango cultivar collection in Guangxi province revealed by ISSR. Molecular Plant Breeding, 3: 829–834.
 
He X.H., Razak S.A., Luo C. (2021): Genetic Diversity Analysis of Mango. The Mango Genome, 75–94.
 
Hidayat A., Rahayu E.S., Abdullah M., Retnoningsih A. (2021): Microsatellites to reveal genetic diversity and to distinguish four mangoes of Tegal District, Central Java, Indonesia. Biodiversitas Journal of Biological Diversity, 22: 3467–3473.
 
Honsho C., Nishiyama K., Eiadthong W., Yonemori K. (2005): Isolation and characterization of new microsatellite markers in mango (Mangifera indica). Molecular Ecology Notes, 5: 152–154. https://doi.org/10.1111/j.1471-8286.2004.00866.x
 
Hu J., Vick B.A. (2003): Target region amplification polymorphism: a novel marker technique for plant genotyping. Plant Molecular Biology Reporter, 21: 289–294. https://doi.org/10.1007/BF02772804
 
Huang X., Zhang X., Huang L., Ma Y., Yin G., Lee S., Zeng J., Liu H. (2014): Genetic diversity of Hemarthria altissima and its related species by EST-SSR and SCoT markers. Biochemical Systematics and Ecology, 57: 338–344. https://doi.org/10.1016/j.bse.2014.09.016
 
Karihaloo J.L., Dwivedi Y.K., Archak S., Gaikwad A.B. (2003): Analysis of genetic diversity of Indian mango cultivars using RAPD markers. The Journal of Horticultural Science and Biotechnology, 78: 285–289. https://doi.org/10.1080/14620316.2003.11511619
 
Kashkush K., Jinggui F., Tomer E., Hillel J., Lavi U. (2001): Cultivar identification and genetic map of mango (Mangifera indica). Euphytica, 122: 129–136. https://doi.org/10.1023/A:1012646331258
 
Kumar H., Narayanaswamy P., Prasad T., Mukunda G.K., Sondur S. (2001). Estimation of genetic diversity of commercial mango (Mangifera indica L.) cultivars using RAPD markers. The Journal of Horticultural Science and Biotechnology, 76: 529–533.
 
Lal S., Singh A.K., Singh S.K., Srivastav M., Singh B.P., Sharma N., Singh N.K. (2017): Association analysis for pomological traits in mango (Mangifera indica L.) by genic-SSR markers. Trees, 31: 1391–1409.
 
Li G., Quiros C.F. (2001): Sequence-related amplified polymorphism (SRAP), a new marker system based on a simple PCR reaction: its application to mapping and gene tagging in Brassica. Theoretical and Applied Genetics, 103: 455–461. https://doi.org/10.1007/s001220100570
 
Litz R.E. (2004): Biotechnology and mango improvement. Acta Horticulturae (ISHS), 645: 85–92. https://doi.org/10.17660/ActaHortic.2004.645.5
 
López-Valenzuela J.A., Martinez O., Paredes-Lopez O. (1997): Geographic differentiation and embryo type identification in Mangifera indica L. cultivars using RAPD markers. HortScience, 32: 1105–1108. https://doi.org/10.21273/HORTSCI.32.6.1105
 
Luo C., He X.H., Chen H., Hu Y., Ou S.J. (2012): Genetic relationship and diversity of Mangifera indica L.: revealed through SCoT analysis. Genetic Resources and Crop Evolution, 59: 1505–1515. https://doi.org/10.1007/s10722-011-9779-1
 
Luo C., He X.H., Chen H., Ou S.J., Gao M.P. (2010): Analysis of diversity and relationships among mango cultivars using Start Codon Targeted (SCoT) markers. Biochemical Systematics and Ecology, 38: 1176–1184. https://doi.org/10.1016/j.bse.2010.11.004
 
Luo C., He X.H., Chen H., Ou S.J., Gao M.P., Brown J.S., Tondo C.T., Schnell R.J. (2011): Genetic diversity of mango cultivars estimated using SCoT and ISSR markers. Biochemical Systematics and Ecology, 39: 676–684. https://doi.org/10.1016/j.bse.2011.05.023
 
Mansour A., Ismail O.M., El-Din S.M. (2008): Diversity assessment among mango (Mangifera indica L.) cultivars in Egypt using ISSR and three-primer based RAPD fingerprints. The African Journal of Plant Science and Biotechnology, 2: 87–92.
 
Mansour H., Mekki L.E., Hussein, M.A. (2014): Assessment of genetic diversity and relationships among Egyptian mango (Mangifera indica L.) cultivers grown in Suez Canal and Sinai region using RAPD markers. Pakistan Journal of Biological Sciences, 17: 56–61. https://doi.org/10.3923/pjbs.2014.56.61
 
Mgendi M.G., Manoko M.L., Nyomora A. (2010): Genetic diversity between cultivated and non-cultivated Moringa oleifera Lam. provenances assessed by RAPD markers. Journal of Cell and Molecular Biology, 8: 95–102.
 
Muluvi G.M., Sprent J.I., Soranzo N., Provan J., Odee D., Folkard G., McNicol J.W., Powell W. (1999): Amplified fragment length polymorphism (AFLP) analysis of genetic variation in Moringa oleifera Lam. Molecular Ecology, 8: 463–470. https://doi.org/10.1046/j.1365-294X.1999.00589.x
 
Pandit S.S., Mitra S., Giri A.P., Pujari K.H., Patil B.P., Jambhale N.D., Gupta V.S. (2007): Genetic diversity analysis of mango cultivars using inter simple sequence repeat markers. Current science, 93: 1135–1141.
 
Powell W., Morgante M., Andre C., Hanafey M., Vogel J., Tingey S., Rafalski A. (1996): The comparison of RFLP, RAPD, AFLP and SSR (microsatellite) markers for germplasm analysis. Molecular Breeding, 2: 225–238. https://doi.org/10.1007/BF00564200
 
Rahman M.L., Rabbani M.G., Siddique M.N.A., Rahman M.A., Garvey E.J., Rahaman E.H.M.S. (2007): Molecular characterization of 28 mango germplasm using RAPD. Plant Tissue Culture and Biotechnology, 17: 71–77. https://doi.org/10.3329/ptcb.v17i1.1123
 
Rashed M., Maklad M. (2016): Genetic relationships among six mango (Mangifera indica L.) cultivars using rapd markers. Egyptian Journal of Genetics and Cytology, 45: 105–112. https://doi.org/10.21608/ejgc.2016.9700
 
Razak S.A., Azman N.H.E.N., Ismail S.N., Yusof M.F.M., Ariffin M.A.T., Sabdin Z.H.M., Hassan M.H.M., Nasir K.H., Sani M.A., Abdullah N. (2019): Assessment of diversity and population structure of mango (Mangifera indica L.) germplasm based on microsatellite (SSR) markers. Australian Journal of Crop Science, 13(2): 315–320.
 
Rohlf F.J. (1998): NTSYS-pc Version 2.02 Numerical Taxonomy and Multivariate Analysis System. Applied Biostatistics Inc., Exeter Software,” Setauket, New York, 43.
 
Samal K.C., Jena R.C., Swain S.S., Das B.K., Chand P.K. (2012): Evaluation of genetic diversity among commercial cultivars, hybrids and local mango (Mangifera indica L.) genotypes of India using cumulative RAPD and ISSR markers. Euphytica, 185: 195–213. https://doi.org/10.1007/s10681-011-0522-y
 
Shahlaei A., Torabi S., Khosroshahli M. (2014): Efficacy of SCoT and ISSR markers in assessment of tomato (Lycopersicum esculentum Mill.) genetic diversity. International Journal of Biosciences, 5: 14–22.
 
Schnell R.J., Ronning C.M., Knight R.J. (1995): Identification of cultivars and validation of genetic relationships in Mangifera indica L. using RAPD markers. Theoretical and Applied Genetics, 90: 269–274. https://doi.org/10.1007/BF00222213
 
Singh A., Singh A.K., Singh S.K. (2012): SSR markers reveal genetic diversity in closely related mango hybrids. Indian Journal of Horticulture, 69: 299–305.
 
Srivastav M., Singh S.K., Prakash J., Singh R., Sharma N., Ramchandra S., Devi R., Gupta A., Mahto A.K., Jayaswal P.K., Singh S. (2021): New hyper-variable ssrs for diversity analysis in mango (Mangifera Indica L.). Indian Journal of Genetics and Plant Breeding, 81: 119–126. https://doi.org/10.31742/IJGPB.81.1.13
 
Srivastava N., Bajpai A., Chandra R., Rajan S., Muthukumar M., Srivastava M.K. (2012): Comparison of PCR based marker systems for genetic analysis in different cultivars of mango. Journal of Environmental Biology, 33: 159–166.
 
Surapaneni M., Vemireddy L.R., Begum H., Purushotham R.B., Neetasri C., Nagaraju J., Anwar S.Y., Siddiq E.A. (2013): Population structure and genetic analysis of different utility types of mango (Mangifera indica L.) germplasm of Andhra Pradesh state of India using microsatellite markers. Plant Systematics and Evolution, 299: 1215–1229.
 
Tomar R.S., Gajera H.P., Viradiya R.R., Patel S.V., Golakiya B.A. (2011): Phylogenetic relationship among Mango (Mangifera indica L.) Landraces of Saurashtra based on DNA fingerprinting. Journal of Horticulture and Forestry, 3: 379–385.
 
Tsai C.C., Chen Y.K.H., Chen C.H., Weng I.S., Tsai C.M., Lee S.R., Lin Y.S., Chiang, Y.C. (2013): Cultivar identification and genetic relationship of mango (Mangifera indica) in Taiwan using 37 SSR markers. Scientia Horticulturae, 164: 196–201. https://doi.org/10.1016/j.scienta.2013.09.037
 
Uddin M.S., Cheng Q. (2015): Recent application of biotechniques for the improvement of mango research. In Applied Plant Genomics and Biotechnology, 195–212.
 
Verma S., Rana T.S. (2011): Genetic diversity within and among the wild populations of Murraya koenigii (L.) resources and their potential parents with start codon targeted (SCoT) markers. Genetic Resources and Crop Evolution, 67: 41–58.
 
Zietkiewicz E., Rafalski A., Labuda D. (1994): Genome fingerprinting by simple sequence repeat (SSR)-anchored polymerase chain reaction amplification. Genomics, 20: 176–183. https://doi.org/10.1006/geno.1994.1151
 
download PDF

© 2023 Czech Academy of Agricultural Sciences | Prohlášení o přístupnosti