The occurrence of Fusarium spp. in green Asparagus officinalis L. spears
Due to changes in the climate and the existence of a wide range of Asparagus officinalis L. cultivars, it is necessary to identify their suitability for cultivation in Poland and investigate their susceptibility to infection by the most common pathogens. The identification of the species composition of fungi of the Fusarium genus found in green spears of edible A. officinalis cultivars and the factors contributing to their occurrence will enable the effective protection of these crops. The study was conducted on seven A. officinalis cultivars bred in different countries, which were dioecious cultivars with male and female specimens: ‘Ariane’ (Germany), ‘Cipres’ (France), ‘Eposs’ (Germany), as well as cultivars with male specimens only: ‘Andreas’ (France), ‘Gynlim’, ‘Grolim’ (Netherlands), and ‘Hannibal’ (Germany). The analysis of the composition of the fungi isolated from the green A. officinalis spears showed that most of the isolates belonged to the Fusarium genus (F. culmorum Wm.G. Sm., F. equiseti (Corda) Sacc., F. oxysporum Schltdl., F. proliferatum (Matsush.) Nirenberg ex Gerlach & Nirenberg, F. solani (Mart.) Sacc., and F. fujikuroi Nirenberg). Other fungal species (Alternaria, Botrytis, Cladosporium, Penicillium, and Stemphylium) were rarely isolated. The majority of the Fusarium genus isolates came from the spears of the ‘Ariane’ and ‘Eposs’ cultivars showing disease symptoms and from the spears of the ‘Grolim’ cultivar without showing disease symptoms. The fungi of the Fusarium genus colonised both the spears with and without disease symptoms, but there were always more isolates on the ones with disease symptoms. Fungi of the Fusarium genus occurred more often in the epidermis than in the parenchyma. F. oxysporum was the dominant fungus in the A. officinalis spears under analysis. The number of fungi isolates of the Fusarium genus collected from the green A. officinalis spears tended to increase at the consecutive harvest dates, which means that the spears harvested at the latest date (late June) were the most heavily colonised by fungi. All of the fungi isolates of the Fusarium genus collected from the spears exhibited pathogenicity against A. officinalis plants.
Andrzejak R., Janowska B. (2021): The occurrence of Fusarium species in white spears of Asparagus officinalis. Notulae Botanicae Horti Agrobotanici Cluj-Napoka, 49: 12559.
https://doi.org/10.15835/nbha49412559
Andrzejak R., Werner M. (2006): Grzyby z rodzaju Fusarium uszkadzające wypustki szparagów i ich patogeniczność względem młodych roślin (Fungi from genus Fusarium spp. damaging asparagus spears and their pathogenicity towards young plants). Progress in Plant Protection, 46: 700–703.
Blok W.J., Bollen G.J. (1995): Fungi on the roots an stem bases of asparagus in the Netherlands: Species and Pathogenicity. European Journal of Plant Pathology, 101: 15–24.
https://doi.org/10.1007/BF01876090
Blok W.J., Bollen G.J. (1996): Etiology of asparagus replant-bound early decline. European Journal of Plant Pathology, 102: 87–98.
https://doi.org/10.1007/BF01877119
Borrego-Benjumea A., Basallote-Ureba M.J., Melero-Vara J.M., Abbasi P.A. (2014): Characterization of Fusarium isolates from asparagus fieldsin southwestern Ontario and influence of soil organic amendmentson Fusarium crown and root rot. Phytopathology, 104: 403–415.
https://doi.org/10.1094/PHYTO-08-13-0231-R
Brizuela A.M., de la Lastra E., Marín-Guirao J.I., Gálvez L., de Cara-García M., Capote N., Palmero D. (2020): Fusarium consortium populations associated with Asparagus crop in Spain and their role on field decline syndrome. Journal of Fungi, 6: 336.
https://doi.org/10.3390/jof6040336
Caron M., Plante F., Devaux A., Emond G. (1985): Current status of Fusarium spp. on Asparagus in Quebec. Proceedings of the 6th International Asparagus Symposium, Guelph, Canada: 144–150.
Elmer W.H. (1996): Fusarium fruit rot of pumpkin in Connecticut. Plant Disease, 80: 131–135.
https://doi.org/10.1094/PD-80-0131
Elmer W.H. (2000): Incidence of infection of asparagus spears marketed in Connecticut by Fusarium spp. Plant Disease, 84: 831–834.
https://doi.org/10.1094/PDIS.2000.84.8.831
Elmer W.H. (2015): Management of Fusarium crown and root rot of asparagus. Crop Protection, 73: 2–6.
https://doi.org/10.1016/j.cropro.2014.12.005
Falloon P.G., Fraser H.A. (1991): Control of establishment failures in asparagus (Asparagus officinalis L.) caused Phytophthora rot. New Zealand Journal of Crop and Horticultural Science, 19: 47–52.
https://doi.org/10.1080/01140671.1991.10418105
Fantino M.G. (1990): Research on Asparagus decline in Italy. Acta Horticulturae (ISHS), 271: 291–298.
https://doi.org/10.17660/ActaHortic.1990.271.42
Fiedorow Z. (1996) Virus diseases of asparagus (Asparagus officinalis L.) in Poland. Progress in Plant Protection, 36: 160–161.
Gossmann M., Kleta S., Humpf H.U., Büttner C. (2005): Untersuchungen zum endophytischen Befall von Fusarium proliferatum (Matsushima) Nirenberg in geernteten Stangen von Spargel (Asparagus officinalis L.). Gesunde Pflanzen, 57: 53–58.
https://doi.org/10.1007/s10343-005-0065-z
Grogan R.G., Kimble K.A. (1959): The association of Fusarium wilt with the asparagus decline and replant problem in California. Phytopathology, 49: 122–125.
Guerrero C., Nigh E.L., Stanghellini M.E. (1999): Fusarium oxysporum and F. proliferatum infection of commercial asparagus spears originates from infected crowns in the field. Acta Horticulturae (ISHS), 479: 227–230.
https://doi.org/10.17660/ActaHortic.1999.479.31
Hsu G., Sun S.K. (1969): Stem blight of asparagus in Taiwan. 1. Distribution of the disease and cultural characters and spore germination of the causal organism, Phoma asparagi Sacc. Plant Protection Bulletin Taiwan, 11: 47–60.
Jaspers M.V. (1996): Effect of asparagus virus 2 on yield of Asparagus officinalis. Acta Horticiculturae (ISHS), 415: 383–386.
https://doi.org/10.17660/ActaHortic.1996.415.54
Kalomira E. (2007): Asparagus diseases. The European Journal of Plant Science and Biotechnology, 1: 76–83.
Kathe L., Krämer R., Budahn H., Pillen K., Rabenstein F., Nothnagel T. (2019): Development of a bioassay to assess resistance to Fusarium oxysporum (Schlecht.) in asparagus (Asparagus officinalis L.). Journal of Phytopathology, 167: 558–566.
https://doi.org/10.1111/jph.12845
Knaflewski M. (2005): Uprawa szparaga. Hortpress, Sp. z o.o. Warszawa, Poland.
Knaflewski M., Sadowski C. (1990): Influence of irrigation, fertilization and spacing on the healthiness of asparagus seedlings. Acta Horticulturae (ISHS), 271: 269–270.
https://doi.org/10.17660/ActaHortic.1990.271.38
Krug H. (1999): Seasonal growth and development of asparagus (Asparagus officinalis L.) V. fern ripening and crown activity in open fields. Gartenbauwissenschaft, 64: 165–172.
Kwaśna H., Chełkowski J., Zajkowski P. (1991): Grzyby (Mycota), XXII. Instytut Botaniki PAN: Warszawa-Kraków, Poland, 136.
Leslie J.F., Summerel B.A. (2006): The Fusarium Laboratory Manual. Blackwell Publishing Ltd: Iowa, USA: 387.
Mańka, M. (1989): Patogeniczność wybranych gatunków z rodzaju Fusarium dla siewek zbóż. Rozprawy naukowe 201. Akademia Rolnicza: Poznań, Poland.
Montealegre J.P., Palma M.J. (1994): Etiology of the main postharvest fungal rots in green asparagus in Chile. Fitopatologia, 29: 102–105.
Nothnagel T., Krämer R., Schreyer L., Rabenstein F. (2013): Untersuchungen zum auftreten von braunverfärbungen bei spargel (Asparagus officinalis L.) unter besonderer berücksichtigung des befalls mit Fusarium spp. und viren in spargelanlagen Sachsen-Anhalts. Journal fur Kulturpflanzen, 65: 50–59.
Sadowski C. (1990): The occurrence of Zopfia Rhizophila Rabenh. on asparagus roots in Poland. Acta Horticulturae (ISHS), 271: 377–381.
https://doi.org/10.17660/ActaHortic.1990.271.53
Sonoda T., Uragami A., Kaji K. (1997): Evaluation of Asparagus officinalis cultivars for resistance to stem blight by using a novel inoculation method. HortScience, 32: 1085–1086.
https://doi.org/10.21273/HORTSCI.32.6.1085
Vujanowić V., Hamel Ch., Yergeau E., St-Arnaud M. (2006): Biodiversity and biogeography of Fusarium species from Northeastern North American Asparagus fields based on microbiological and molecular approaches. Microbial Ecology, 51: 242–255.
https://doi.org/10.1007/s00248-005-0046-x
Weber Z., Karolewski L., Irzykowaska L., Knaflewski M., Kosiada T. (2007): Occurrence of Fusarium species in spears of asparagus (Asparagus officinalis). Phytopathologia Polonica, 45: 9–15.
Weber Z., Knaflewski M. (2004): Ocena zdrowotności wypustek dwóch odmian szparaga. Progress of Plant Protection, 44: 1197–1200.
Witaszak N., Lalak-Kańczugowska J., Waśkiewicz A., Stępień Ł. (2020): The impacts of asparagus extract fractions on growth and fumonisins biosynthesis in Fusarium proliferatum. Toxin, 12: 95.
https://doi.org/10.3390/toxins12020095
, Beata Janowska